Receptor-binding capability of pancreatic phospholipase A2is separable from its enzymatic activity
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Enzymatic Activity of a Phospholipase A2: An Electrochemical Approach
A novel approach based on dc and ac cyclic voltammetric techniques to evaluate interfacial enzymatic activity is presented. As an illustrative example, the hydrolytic effect of phospholipase A2 (PLA2) on an L-R-dioleoylphosphatidylcholine (DOPC) monolayer adsorbed onto a mercury electrode surface has been studied and kinetic parameters have been evaluated. The enzymatic activity obtained in thi...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1993
ISSN: 0014-5793
DOI: 10.1016/0014-5793(93)80149-o